Multilocus sequence typing and FlaA sequencing reveal the genetic stability of Campylobacter jejuni enrichment during coculture with Acanthamoeba polyphaga.
نویسندگان
چکیده
Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.
منابع مشابه
Amoebae as Hosts and Vectors for Spread of Campylobacter jejuni
Olofsson, J. 2015. Amoebae as Hosts and Vectors for Spread of Campylobacter jejuni. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1115. 50 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9276-2. Campylobacter jejuni is the leading bacterial cause of gastrointestinal diarrheal disease in humans worldwide. This zoonotic pathogen has a complex e...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 79 7 شماره
صفحات -
تاریخ انتشار 2013